Orthogonal tRNA/aaRS Pair Identification
Identification of functionally independent tRNA/aaRS pairs that minimize cross-reactivity with host systems.
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Modular Engineering Platform
Customizable Service Packages
In-House Library Generation
Workflow Integration
High-Fidelity Quality Control
Protheragen is a leading provider of preclinical services and a pioneering biotech company focused on enabling site-specific incorporation of non-canonical amino acids (ncAAs) through high-throughput orthogonal tRNA/aaRS pair development, offering powerful tools for pharmaceutical R&D, synthetic biology, diagnostics, and more.
With a multidisciplinary team and a strong foundation in molecular biology, protein chemistry, and genome engineering, we offer scalable and modular solutions to support academic and industrial partners. Our goal is to advance genetic code expansion technologies and provide robust tools for the development of next-generation biomolecules.
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Identification of functionally independent tRNA/aaRS pairs that minimize cross-reactivity with host systems.
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Evaluation of orthogonality and incorporation fidelity in cell-free systems to support downstream in vivo studies.
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Implementation of ncAA incorporation strategies in E. coli, yeast, and mammalian systems to support functional studies and product development.
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Tailored Solutions Powered by Deep tRNA Engineering
Standard, mutant, and unnatural tRNAs are available, supported by in-house oligo synthesis and gene optimization services.
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We offer both natural and unnatural chemical modifications, along with site-specific editing for functional enhancement.
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Includes integrity assessment, purity evaluation, concentration analysis, and detection/removal of genomic DNA contamination.
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Our purification options include gel-based, affinity-tag, and native purification for both prokaryotic and eukaryotic tRNAs.
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Enables the development of advanced therapeutic peptides, proteins, and antibodies by integrating non-canonical amino acids to improve efficacy, specificity, and pharmacological properties across diverse disease areas.
Expands the chemical and functional diversity of biological systems through site-specific ncAA incorporation, supporting the design of novel biomolecules with enhanced or unnatural functionalities.
Facilitates the creation of sensitive and customizable biosensors using ncAA-modified peptides and proteins for precise detection and high-throughput analytical applications.
Our in vitro expression kits are optimized for the rapid evaluation of orthogonal tRNA/aaRS systems and ncAA incorporation. These kits enable efficient protein synthesis in cell-free systems, facilitating early-stage screening and functional validation of engineered biomolecules.
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We provide a comprehensive portfolio of high-quality oligonucleotides and synthetic peptides, including custom-modified and stable formats. These reagents are ideal for translation studies, peptide engineering, RNA labeling, and biosensor development.
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Our plasmid collection includes specialized vectors encoding orthogonal tRNAs, engineered aminoacyl-tRNA synthetases, and validated tRNA/aaRS pairs. Designed for high-efficiency expression in bacterial, yeast, or mammalian systems.
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A specialized platform advancing genetic code expansion through orthogonal tRNA/aaRS technologies, enabling precise ncAA incorporation for biotherapeutic development, synthetic biology, and diagnostics.